Recombinant Protein Production in E. coli Using the phoA Expression System
نویسندگان
چکیده
Auto-inducible promoter systems have been reported to increase soluble product formation in the periplasm of E. coli compared inducer-dependent systems. In this study, we investigated phosphate (PO4)-sensitive phoA expression system (pAT) for production a recombinant model antigen-binding fragment (Fab) detail. We explored impact non-limiting and limiting PO4 conditions on strain physiology as well Fab productivity. different methods extracellular detection, identifying automated colorimetric measurement be most suitable at-line monitoring. showed that limitation boosts phoA-based gene expression, however, was already formed at conditions, indicating leaky expression. Furthermore, cultivation under caused physiological changes ultimately resulting metabolic breakdown starvation. Finally, give recommendations process optimization with system. summary, our study provides very detailed information system, thus extending existing knowledge underlines its high potential successful periplasmic products coli.
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ژورنال
عنوان ژورنال: Fermentation
سال: 2022
ISSN: ['2311-5637']
DOI: https://doi.org/10.3390/fermentation8040181